ISO／DIS 19574：2020.docx

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1、DRAFTINTERNATIONA1STANDARDISO/DIS19574ISOTC216Votingbeginson:2023-01-10Secretariat:UNEVotingterminateson:2023-04-03FootwearandfootwearcomponentsQua1itativetestmethodtoassessantifunga1activity(growthtest)ChaussuresetComposantsdeChaussuresMethoded,essaidecroissancequa1itativepoureva1uer1esproprietesQn

2、tifongiquescontre1esmicrochampignonsfi1amenteuxICS:61.060THISDOCUMENTISADRAFTCIRCU1ATEDFORCOMMENTANDAPPROVA1.ITISTHEREFORESUBJECTTOCHANGEANDMAYNOTBEREFERREDTOSANINTERNATIONA1STANDARDUNTI1PUB1ISHEDASSUCH.INADDITIONTOTHEIREVA1UATIONASBEINGACCEPTAB1EFORINDUSTRIA1TECHNO1OGICA1,COMMERCIA1ANDUSERPURPOSES.

3、DRAFTINTERNATIONA1STANDARDSMAYONOCCASIONHAVETOBECONSIDEREDINTHE1IGHTOFTHEIRPOTENTIA1TOBECOMESTANDARDSTOWHICHREFERENCEMAYBEMADEINNATIONA1REGU1ATIONS.RECIPIENTSOFTHISDRAFTAREINVITEDTOSUBMIT.WITHTHEIRCOMMENTS.NOTIFICATIONOFANYRE1EVANTPATENTRIGHTSOFWHICHTHEYAREAWAREANDTOPROVIDESUPPORTINGDOCUMENTATION.Th

4、isdocumentiscircu1atedasreceivedfromthecommitteesecretariat.ISO/CENPARA11E1PROCESSINGReferencenumberISO/DIS19574:2023(E)ISO2023COPYRIGHTPROTECTEDDOCUMENTISO2023A11rightsreserved.Un1essotherwisespecied,orrequiredinthecontextofitsimp1ementation,nopartofthispub1icationmaybereproducedoruti1izedotherwise

5、inanyformorbyanymeans,e1ectronicormechanica1,inc1udingphotocopying,orpostingontheinternetoranintranet,withoutpriorwrittenpermission.PermissioncanberequestedfromeitherISOattheaddressbe1owor!SO,smemberbodyinthecountryoftherequesterISOcopyrightofficeCP401Ch.deB1andonnet8CH-1214VernieGenevaPhone:*412274

6、90111Fax:41227490947Emai1:copyrightiso.orgWebsite:www.iso.orgPub1ishedinSwitzer1andPageContentsForeword1Scope145Princip1eSafety226APParatuS27Reagentsandcu1turemedium3372Water37.3Ma1textractagar(MEA)medium37.3.1Composition37.3.2Preparation37.4Physio1ogica1sa1ine(sodiumch1orideso1ution)47.4.1Compositi

7、on47.4.2Preparation47.5Wettingagent(nonionicsurfactant)47.6Bufferso1ution47.6.1Bufferstock47.6.2Preparationofbufferstock47.6.3PreparationOfbufferso1ution4849Preparationoftestsporesuspension10Preparationoftstspecimens10.2Testandcontro1specimens10.3Pre-treatmentoftestandcontro1specimens61111.1Inocu1at

8、ionofso1idiedagarp1ates11.2 Arrangementoftestandcontro1specimens11.3 Inocu1ationoftestandcontro1specimens77711.5Viabi1itycontro1ofthespores711.6Assessmentofmicro-funga1growth712Expressionofresu1ts713Testrenort8Bib1iography93TermsanddenitionsForewordISO(theInternationa1OrganizationforStandardization)

9、isawor1dwidefederationofnationa1standardsbodies(ISOmemberbodies).TheworkofpreparingInternationa1Standardsisnorma11ycarriedoutthroughISOtechnica1committees.Eachmemberbodyinterestedinasubjectforwhichatechnica1committeehasbeenestab1ishedhastherighttoberepresentedonthatcommittee,internationa1organizatio

10、ns,governmenta1andnon-governmenta1,in1iaisonwithISO,a1sotakepartinthework.ISOco11aboratesc1ose1ywiththeInternationa1E1ectrotechnica1Commission(IEC)ona11mattersofe1ectrotechnica1standardization.Theproceduresusedtodeve1opthisdocumentandthoseintendedforitsfurthermaintenancearedescribedinthe1SOIECDirect

11、ives,Part1.Inparticu1arthedifferentapprova1criterianeededforthedifferenttypesofISOdocumentsshou1dbenoted.Thisdocumentwasdraftedinaccordancewiththeeditoria1ru1esoftheISO/IECDirectives,Part2(seeWWW,isoQigdiecUves).Attentionisdrawntothepossibi1itythatsomeofthee1ementsofthisdocumentmaybethesubjectofpate

12、ntrights.ISOsha11notbehe1dresponsib1eforidentifyinganyora11suchpatentrights.Detai1sofanypatentrightsidentifiedduringthedeve1opmentofthedocumentwi11beintheIntroductionand/orontheISO1istofpatentdec1arationsreceived(seeWWW.iso.org/patents).Anytradenameusedinthisdocumentisinformationgivenfortheconvenien

13、ceofusersanddoesnotconstituteanendorsement.Foranexp1anationonthevo1untarynatureofstandards,themeaningofISOspecifictermsandexpressionsre1atedtoconformityassessment,aswe11asinformationaboutISOsadherencetotheWor1dTradeOrganization(WTO)princip1esintheTechnica1BarrierstoTrade(TBT)seethefo11owingUR1:www.i

14、so.org/iso/foreword.htm1ThisdocumentwaspreparedbyTechnica1Committee1SOTC216,Footwear.FootwearandfootwearcomponentsQua1itativetestmethodtoassessantifunga1activity(growthtest)CAUTIONTestmethodsspecifiedhereinrequiretheuseofmicro-fungi.Thesetestsareon1ytobecarriedoutinfaci1itieswithcontainmenttechnique

15、sforhand1ingmicroorganismsandbypersonswithtrainingandexperienceintheuseofmicrobio1ogica1techniques.1 ScopeThisInternationa1Standardspecifiesatestmethod(growthtest)forqua1itativeeva1uationoftheantifunga1activityoffootwearandfootwearcomponentsduetotheactionOffi1amentousmicro-fungi.ThisInternationa1Standardisapp1icab1eon1ytofootwearandcomponentsthatc1aimtohaveantifunga1(antimycotic)orantimicrobia1treatmenteffects.2 NormativereferencesThefo11owingdocumentsarereferred