双特异性磷酸酶13抗体纯化方法.docx

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1、英文名称DUSPI3中文名称双特异性磷酸酶抗体13抗体别名BEDP;Branchingenzymeinteractingdua1specificityproteinphosphatase;Dua1specificityphosphatase13;Dua1specificityphosphataseSKRP4;DUSP13;F1J32450;DS13A_H1MAN;MDSP;Musc1erestricteddua1specificityphosphatase:SKRP4;Tcstisandske1eta1musc1especificDSP;TMDP.规格0.Im10.2m1研究领域细胞生物免疫学

2、发育生物学信号转导激酶和磷酸酶抗体来源Rabbit克隆类型Po1yc1ona1交叉反应Human,Mouse,Rat,产品应用WB=I:100-500E1ISA=I:500-1000IP=I:20-100IHC-P=I:100-500IIIC-F=I:100-500ICC=I:100-500IF=I:100-500双特异性磷酸酶抗体13抗体(石蜡切片需做抗原修复)notyettestedinotherapp1ications.量状度原型法液件I1ma子疫存得Op分性浓免亚纯储保diIutions/concentrationsshou1dbedeterminedbytheenduser.2IkD

3、a1yophi1izedor1iquid1mg/1m1K1HconjugatedsyntheticpeptidederivedfromhumanDUSPI3IgGaffinitypurifiedbyProteinAPreservative:15mMSodiumAzide,Constituents:1%BSA,0.O1MPBS,pH7.4Storeat-200Cforoneyear.Avoidrepeatedfreeze/thawcyc1es.The1yophi1izedantibodyisstab1eatroomtemperatureforat1eastonemonthandforgreate

4、rthanayearwhenkeptat-20oC.Whenreconstitutedinsteri1epH7.40.O1MPBSordi1uentofantibodytheantibodyisstab1eforat1easttwoweeksat2-4C.双特异性磷酸酶抗体13抗体产品介绍background:Membersoftheprotein-tyrosinephosphatasesuperfami1ycooperatewithproteinkinasestoregu1atece11pro1iferationanddifferentiation.Thissuperfami1yissepa

5、ratedintotwofami1iesbasedonthesubstratethatisdephosphory1ated.Onefami1y,thedua1specificityphosphatases(DSPs)actsonbothphosphotyrosineandphosphoserinethreonineresidues.Thisgeneencodesdifferentbutre1atedDSPproteinsthroughtheuseofnon-over1appingopenreadingframes,a1ternatesp1icing,andpresumeddifferenttr

6、anscriptionpromoters.Expressionofthedistinctproteinsfromthisgenehasbeenfoundtobetissuespecificandtheproteinsmaybeinvo1vedinpostnata1deve1opmentofspecifictissues.AproteinencodedbytheupstreamORFwasfoundinske1eta1musc1e,whereastheencodedproteinfromthedownstreamORFwasfoundon1yintestis.Inmouse,asimi1arpa

7、tternofexpressionwasfound.Mu1tip1ea1ternative1ysp1icedtranscriptvariantsweredescribed,butthefu11-1engthsequenceofon1ysomeweredetermined,providedbyRefSeq,Ju12008Function:DUSP13maybeinvo1vedintheregu1ationofmeiosisand/ordifferentiationoftesticu1argermce11sduringspermatogenesis.Itexhibitsintrinsicphosp

8、hataseactivitytowardsbothphospho-sery1/threony1and-tyrosy1residuesofmye1inbasicprotein.Subunit:InteractswithMAP3K5ASK1;maycompetewithAKT1preventingMAP3K5ASK1phosphory1ationbyAKT1.Subce11u1ar1ocation:Cytop1asm.TissueSpecificity:Ske1eta1musc1especific.Simi1arity:Be1ongstotheprotein-tyrosinephosphatase

9、fami1y.Non-receptorc1assdua1specificitysubfami1y.Contains1tyrosine-proteinphosphatasedomain.GeneID:51207Database1inks:EntrezGene:51207HumanEntrezGene:27389MouseEntrezGene:361002RatOmim:613191HumanSwissProt:Q9UII6HumanSwissProt:Q9QYJ7MouseImportantNote:Thisproductassupp1iedisintendedforresearchuseon1y,notforuseinhuman,therapeuticordiagnosticapp1ications.