20140612田家泽本科论文终稿修订版.docx
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1、石河子大学动物科技学院本科毕业论文中药复方宫炎净不同溶剂粗提物对小鼠抗炎活性的研究院(系):动物科技学院专业:动物医学学号:2010513124姓名:田家泽指导教师:罗燕(副教授)新疆石河子2014. 6摘要目的:通过中药复方宫炎净不同溶剂粗提物对小鼠抗炎活性的研究,筛选最佳提取溶剂及其抗炎剂量。方法:利用渗漉法对中药复方宫炎净进行粗提;取110只21日龄健康雌性小鼠随机均分为11组,分别给予生理盐水、地塞米松和高、中、低剂量的石油隧、70%乙醇、水粗提物,连续给药4天后,用二甲苯致炎,测定小鼠耳廓肿胀率;相同方法分组、给药,在最后一次给药30min后,每只小鼠右后足跖注射0.03 mLl%鸡
2、蛋清,4 h后处死,取两后足跖称重计算足趾肿胀率;相同方法分组、给药,最后一次给药1 h后,每只小鼠尾静脉注射0.2 mL质量分数为1%的伊文思兰溶液,然后腹腔注射0.7%的冰醋酸,于590 nm波长处测腹腔液0D值,测定毛细血管通透性;相同方法分组,肩胛部皮下植入棉球,次日起每天给药一次,连续20天,最后一次给药lh后处死小鼠,取出棉球及周围肉芽肿烘干称重。结果:乙醇粗提物各剂量组的耳廓肿胀率、足趾肿胀率、腹腔液0D值、肉芽组织重极显著低于生理盐水组和石油隧、水粗提物各组(P0.01),且乙醇中剂量效果最佳。结论:70%乙醇可作为提取宫炎净有效成分的最佳粗提溶剂,并且以中剂量为最佳抗炎剂量。
3、关键词:中药复方宫炎净;粗提物;毛细血管通透性;耳廓肿胀率;足趾肿胀率;肉芽组织重AbstractObjective To screen the best extraction solvent and anti-inflammatory dose,study the impact that different solvent extracts of traditional Chinese medicinecompound Gongyanping to the anti-inflammatory activity in mice. Methods The21-day-old female mic
4、e of 110 were randomly divided into 11 groups, which weregiven saline, dexamethasone, and high, medium, low doses of petroleum ether,ethanol and water extract and inflammation with xylene after 4 days to determinatethe swelling rate of mouse ear. Grouped and administered by the above method, eachmou
5、se inject 0.03 mLl% egg-induced inflammation on the right rear toe after the lastadministration of 30min. then take the two right rear toe and weight to calculate therate of swollen toe after 4h deadly; Grouped and administered by the above method,intraperitoneal inject the 0.7% acetic acid-induced
6、inflammation after the last dose,measuring the OD values of peritoneal fluid at a wavelength of 590nm and thecapillary permeability; Grouped by the above method, Cut the scapular skin andsuture after putting cotton implanted subcutaneous; Start from the second day,administered once daily for 20 days
7、 and the last administration the mice weresacrificed after lh. Lastly, remove the cotton ball and surrounding granulation tissue.At the same time, drying and weighted the tissue granulation. Results Ear swellingrate of ethanol extract objects in each dose group, peritoneal fluid OD values,granulatio
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