CLASS-VP定量测定操作流程（内标法）.docx

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1、C1ASS-VP定量测定操作流程（内标法）1、新建方法一设定仪器参数一方法储存，方法名为IS-test.met2、指定色谱峰，选择DefinePeakS-A指定色谱峰区域一A出现如下窗口设定如下：点击OK3、编辑峰表（选择MethOd/peak/groups）设定如下：（内标法，3点校正，3号峰为内标峰；如为外标法，则无内标峰,不需指定表中ISTDID#）方法储存4、通过后处理方式建立标准曲线（多点校正时需建立序列表）4-1、建立序列表Fi1e/sequence/new点击OK储存序列表：fi1e/sequence/saveas序列表文件名为IS-test.seq点击0K4-2、运行seque

2、nce(sequence/process)ProcessSequence点击Start5、查看校准曲线(MethOd/reviewca1ibration)3;Iristrujnent1Method：IS-tet.metData：1C-UV_1eue1_3_pacT.dat-ReviewPeakCa1ibration-DetectorA二IFi1eEditViewMethodDataSequenceAna1ysisContro1ReportsWindowHe1p-f1-IffI1M3隹僮I量图日嘲阂酬匾网国|，|aIRIJj日回1eve1AreaRa1ioAmountRatioRF1astAre

3、aRatioRepStDevRep%RS10.7008361014.268721.33629128.9801133.55382205.62775Interna1StandardCurve(3)OHeinUnO4AverageRF:9.62551RFStDev:4.35646RFRSD:45.2596Sca1ing:None1SQWeighting:NoneForceThroughZero:OffRep1icateMode:Rep1ace1inearFitax+ba=3.53182b=7.41793Goodnessoffit(r2):0.999442同川T1a恒恒IJNW4卜汁M册七I川用同刈T

4、退!刈钊童念I剧园跖卜同ForHe1p,pressF1MIN6、计算未知样品浓度6-1、后处理方式6-1-1打开未知样品数据文件(Fi1edataOPen)Options-OpenWithMethodResu1ts:OpenWithConfiguration1astI1C-UV_1eve1_Unk1_pacIA11Fi1es)文侔名世IJ:文佯类型QJ：Findfi1esthatmatchthesecriteria:S.amp1eID:Ana1yst:Acguired:Modified:anytimeanytimeFindNowNewSearchI点击open6-1-2查看结果(reports

5、/VieW/Interna1standard(DetA)Instrument1Method：ISetest.metData：1C-UV-1eve1_1-pac.dat-ViewMethodCustomReport二1Fi1eEditViewMethodDataSequenceAna1ysisContro1ReportsWindowHe1p-g-|t9|道回口IDetectorATi和隹僮IIa1阖圜富I富I日甯I及图I回I喇画明。IRI咧曾Page1of1ShimadzuC1ASS-VPV5.03Interna1StandardReport,MethodName:C:C1ASS-VPMETHO

6、DSMS-IestjnetDataName:C:C1ASS-VPDATAMC-UV_1eve1_1_pacat0100-RetentionTimeISDconcentrationIntegrationCodes5a4.7456b5.4187IS6.46114406510.000CA11646405.000CA12055620.000CA1VBBBBVBBDetectorA(254ju)Pk#NameRetentionTimeArea6-2、实时采集未知样品数据并计算结果6-2-1未知样品进样(ControI/Sing1enm)点击Start,进样，采集数据6-2-2查看结果(reports/view/Interna1standard(DetA)